"This book describes current methods for the identification and characterization of the major hallmarks of senescent cells. Found insideThis Open Access textbook provides students and researchers in the life sciences with essential practical information on how to quantitatively analyze data images. Moreover, properties of gold nanostars as contrast agents for in vivo imaging and interaction of GNS with cells are also discussed in this Brief. This volume provides an overview of commonly used methods and protocols for cell fitness indicators. Found inside – Page 403The chromatin in the nucleus was stained by incubation in PBS containing 1 μM ... Quantitative evaluation of the results was performed with ImageJ software. Progress in Optics Volume 45. Found inside – Page 902ImageJ automatic nucleus counter within the particle analysis plugin was used to process the images. Percentage of viable cells was calculated with respect ... Found insideThis book articulates the strong need for biologists and computer vision experts to collaborate to overcome the limits of human visual perception, and devotes a chapter each to the major steps in analyzing microscopy images, such as ... Based on course notes of SIGGRAPH course teaching techniques for real-time rendering of volumetric data and effects; covers both applications in scientific visualization and real-time rendering. Found inside – Page 32To analyze the paraventricular nucleus (PVN) and periventricular nucleus, ... The ImageJ plugin Cell Counter was used to count pSTAT3-positive cells. Found inside – Page 346Laser illumination can be regulated with a custom - built , programmable electronic counter , which is triggered by the ... Plug - in for ImageJ ( http://rsb.info.nih.gov/ij/ ) specifically developed to meet our demands ( information available from the ... Presented in a clear and easy-to-understand style Makes statistics tangible and enjoyable for even the most hesitant student Features multiple formulas to facilitate comprehension Written by of the foremost entomologists of his generation ... Enough data are now available so that the Blue Brain Project can realistically tackle a model of the sensory column in rat. Few will deny however, that a comprehensive framework of the function and structure of columns has remained elusive. This volume explores open-source based image analysis techniques to provide a state-of-the-art collection of workflows covering current bioimage analysis problematics, including colocalization, particle counting, 3D structural analysis, ... Analysis, visualization, and management of these time series data sets is a growing field of research that has become increasingly important both for experimentalists and theorists interested in brain function. The principle philosophy of this compilation is that practical application is everything; therefore, both classical and modern methods are presented in detail, with examples involving conventional, medium- and high-pressure techniques. Those new to bioscience research as well as experienced practitioners will find that Mouton’s explanations are the perfect companion for stereology courses and workshops. This volume provides methods and approaches to study genetic and environmental regulatory controls on odontogenesis. Found inside – Page 114For the co - localization analysis , background was subtracted using WCIF ImageJ , nuclei with adequate HA staining were identified using the Nucleus Counter plug - in and were then processed with the Intensity Correlation Analysis plug - in ... The Image Processing Handbook, Fifth Edition is fully updated and expanded to reflect the latest developments in the field. Written by an expert with unequalled experience and authority, it offers clea This volume provides an up-to-date compilation of current methodological approaches utilized for the exploration of nucleolar structure and function. This volume covers broad aspects of cell expansion in three different cell types: root hairs, pollen tubes, and hypocothyl cells. Found inside – Page 217Dead cells were then counted using the plug-in 'nucleus counter'. ... This step was done using the open source macro of ImageJ. In this routine, the red and ... This volume presents a comprehensive overview of the wide range of functions recently identified for NE proteins and their relevance in cancer biology, providing molecular mechanisms and evidence of their value as prognostic and diagnostic ... This volume explores the latest updates on microscopy approaches and techniques used by scientists studying in the field of gene expression imaging. These updates cover the technical design of the experiments and the expected outcomes. This volume provides an overview of advanced fluorescence microscopy, covering a broad range of methods. Each chapter focuses on a different method and provides a practical guide for application in biological systems. Practical and adaptable, Mammalian Cell Viability: Methods and Protocols serves as a self-contained laboratory manual useful to both experienced researchers and those new to this incredibly important and influential field. A reliable guide for newcomers to the field as well as experienced scientists, this is a unique resource for anyone interested in applying the power of twenty-first-century genomics to epigenetic studies. This volume brings experts in high-resolution optical microscopy applications in neuroscience and cell biology together to document the state of the art. This book brings a broad review of recent global developments in theory, instrumentation, and practical applications of electron microscopy. Found inside – Page 54Nuclei were stained with 49,6-diamidino-2-phenylindole (Roche). ... numbers were determined using the nucleus counter macro of the ImageJ 1.46e software. This first book on processing and analysis of 3D images of materials structures describes how to develop and apply efficient and versatile tools for geometric analysis and contains a detailed description of the basics of 3d image analysis. The book provides a good introduction for beginners before looking in greater detail at more advanced material in selected areas. The volume highlights the importance of technique in gathering quantitative information. In The Inflammasome: Methods and Protocols, expert researchers in the field detail methods and protocols to study multiple aspects of inflammasome function. Found inside – Page 24Cell nuclei were counter-stained with Hoechst dye (1:500, 10–25 min). ... Antibody internalization was quantified (ImageJ) as the increase in intracellular ... "This book examines the application of artificial intelligence in medical imaging diagnostics"-- This volume of Methods in Cell Biology is the 3e, and provides comprehensive compendia of laboratory protocols and reviews covering all the new methods developed since 2004. Offers step-by-step protocols, troubleshooting tips and more. Ever since their discovery more than 150 years ago, astrocytes have proved to be something of an enigma. Found inside – Page 82Protein bands were quantified using ImageJ software. ... 6 -diamidino-2-phenylindole (DAPI) to counter stain DNA for nucleus visualization. This volume details experimental approaches used to investigate phagocytosis and phagosome maturation. Written by leading experts in the field, this book presents a unique practical perspective of state-of-the-art microscope image processing and the development of specialized algorithms. Thoroughly updated throughout, this companion to Brenner & Rector’s The Kidney, 9th Edition provides the newest information regarding categorizing and classification of diseases and describes how this relates to the various morphological ... -- Activation and regulation of reserve liver progenitor cells -- Adult cardiac-derived stem cells: differentiation and survival regulators -- TGF-Beta1 regulates differentiation of bone marrow mesenchymal stem cells -- Maternal intake of ... Found inside – Page 60Outlines of the brainstem nuclei were determined in MAP2 and CSPG overview ... CA, USA) and then counted with the aid of ImageJ plug-in “cell counter” (Kurt ... This book provides a concise set of protocols for assessing basic neutrophil functions, investigating specialized areas in neutrophil research, and completing step-by-step diagnostic assays of common neutrophil disorders. This book constitutes the thoroughly refereed proceedings of the 13th International Conference on Image Analysis and Recognition, ICIAR 2016, held in Póvoa de Varzim, Portugal, in July 2016. The text is supported by practical examples and carefully constructed chapter-ending exercises drawn from the authors' years of teaching experience, including easily adaptable Java code and completely worked out examples. Found insideThis volume covers the topics presented at the 3rd International Conference on Tumor Microenvironment and Cellular Stress by an international community of researchers. In den letzten Jahren hat sich der Workshop "Bildverarbeitung für die Medizin" durch erfolgreiche Veranstaltungen etabliert. 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